Figure 7

Comparison of positive assays using Methods 1, 2 and 3. The number of time-points with assays that were all positive (3/3), all negative (0/3) or 1/3 or 2/3 positive using Methods 1, 2 or 3 are expressed as percent of total number. For CSP and AMA1, the numbers of time-points that were 3/3 positive was highest using Method 1 and dropped with Method 2 and was lowest with Method 3. Conversely, the number of time-points that were all 3/3 negative was lowest with Method 1 and rose with Method 2 and was highest with Method 3. When CSP and AMA1 were combined, Method 1 identified more 3/3 positive or 3/3 negative (0/3 positive) time-points than Methods 2 or 3.