var gene transcription and PfEMP1 expression in the rosetting and cytoadhesive Plasmodium falciparum clone FCR3S1.2

Table 1 Summary of adhesive characteristics of pRBC of the P. falciparu m clone FCR3S1.2

*in vivo*
Intravascular sequestration in Sprague Dawley rats^a	+
Intravascular sequestration in Macaca fascicularis^a	+
*in vitro*
Rosetting (% rosetting pRBC)^b	80-90%
Giant-rosetting/auto-agglutination^b	+
Soluble heparin^c	90%
Blood Group A^c	90%
Ig-binding anti-Ig^c	96%
Ig-binding anti-IgM^c	90%
Ig-binding anti-IgG^c	12-20%
HUVEC^d	1,200-1,600
Melanoma cells^d	400-500
CHO-CD36^d	200-300
CHO-ICAM1^d	40 ± 12
CHO cells^d	6 ± 3
L-cells (PECAM-1/CD31)^d	390 ± 28
L-cells^d	5
sPECAM-1/CD31^d	183 ± 31
TSP^e	-
CSA^e	-
Placenta^f	0

a) Rats or macaques were administrated with ^{99 m}Technetium-labeled pRBC of the FCR3S1.2 clone by injection into the tail vein (rats) or Vena saphena magna (macaques). The animals were left for 60 min after which sequestration was measured in a triple headed-gamma camera [20, 44]
b) Rosetting rates are expressed as the range of percent rosetting trophozoite-pRBC [33].
c) Percentage of late stage pRBC showing surface fluorescence when incubated with antibodies to human non-immune Ig, IgM or IgG, or heparin, or the blood group ABO antigens [33]
d) Number of pRBC bound per 100 cells [17, 33]
e) Number of late stage pRBCs bound to thrombospondin-coated plastic (50 mg/ml)
f) Number of IEs bound to 1 mm² of placental tissue

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