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Table 1 Species prevalence using microscopy compared to different detection methods, paired analysis

From: Considerations on the use of nucleic acid-based amplification for malaria parasite detection

Species

Micros.

SMRU

Nested PCR

Multiplex PCR MSHR-F

  

MNHN-B

SMRU-B

SMRU-F

 
 

N (%)

N (%)

P

N (%)

P

N (%)

P

N (%)

P

Pf

164 (40)

215 (52)

0.001

163 (39)

1.000

170 (41)

0.180

193 (47)

0.001

Pv

173 (42)

193 (47)

0.001

174 (42)

1.000

175 (42)

0.832

169 (41)

0.626

Mixed

29 (7)

72 (17)

0.001

29 (7)

1.000

43 (10)

1.000

24 (6)

0.635

Neg

100 (24)

74 (18)

0.001

104 (25)

0.424

109 (26)

0.022

73 (18)

0.001

  1. Note: N = Sample numbers = N; P = statistical significance (Micros. SMRU used as the comparator). Pf = P. falciparum and Pv = P. vivax (for both including mixed infections); Mixed = Pf+Pv or Pf+Po (P. ovale); Neg = negative. Micros. = Microscopic examination; PCR: Polymerase Chain reaction; MNHN: Museum National d'Histoire Naturelle; SMRU: Shoklo Malaria Research Unit; MSHR: Menzies School of Health Research. B: whole blood sample; F: blood spotted on filter paper