Figure 5

Binding inhibition activity of anti-VAR2CSA plasma. Binding inhibitory activity of anti-VAR2CSA plasma were tested in an in vitro binding assay with four different CSA-binding parasite lines and a CD36 binding control parasite line. The binding inhibitory activity was tested in a CSA-binding assay for the CSA-binding parasites and in a CD36 binding assay for the CD36 binding parasite. All anti-VAR2CSA plasma were tested as a pool of the three rats in each group, except rat #1 anti-DBL4 plasma was tested on its own because the other two rat plasma did not react by flow cytometry. A mixture of plasma against five 7G8-DBL domains (DBL1, DBL3, DBL4, DBL5, and DBL6) was also tested against the homologous 7G8-CSA parasite line. The extent of binding inhibition was expressed relative to the pool of rat immune anti-IT4var22 DBL3. The mean and range of binding inhibition from duplicate experiments is shown.