Figure 2
Gene networks involved in the metabolic adaptation to hyperoxia in tightly synchronized P. falciparum cultures. Using PS 6.0™ software and microarray expression data, gene networks were built and genes modulated in response to hyperoxia are represented. (A) Representation of metabolic interrelations related to adaptative hyperoxia exposure. Energetic metabolism: aspartate carbamoyltransferase (P13_0240), carbamoyl-phosphate synthetase (PF13_0044), glutamine-fructose-6-phosphate transaminase (PF10_0245), glucose-6-phosphate dehydrogenase (PF14_0511), acetyl-CoA synthetase (PFF1350c), gapdh (PF14_0598) - Signal transduction: regulatory sub-unit of cAMP-dependent protein kinase (PFL1110c), catalytic sub-unit of cAMP-dependent protein kinase (PFI1685w), ser thr protein kinase (PF13_0085) - Translation: citrate synthase (PF10_0218), translation elongation factor 1 alpha 1 (PF13_0305), proteasome 26S subunit (MAL13P1.343), dihydrolipoamide dehydrogenase (PFL1550w), translation elongation factor 2 (PF14_0486), adaptor-related protein complex 1 (PF13_0062), polymerase RNA I (PF11_0358) - DNA repair: DNA primase (PF14_0366 and PFI0530c), rpa1 (PFI0235w) - Protein folding: ferredoxin reductase (PF11_0407), Hsp10 (PFL0740c), Hsp60 (PF10_0153), Hsp70 (PF11_0351 and PF08_0054), Hsp90 (PFL1070c and PF07_0029), prohibitin (PF08_0006), DnaJ (PFF1415c). (B) Representation of ATP-dependent gene sub-networks altered in hyperoxic conditions. V-type ATPase: V-type ATPase putative (MAL13P1.271), vacuolar ATP synthase subunit h putative (PF13_0034), vacuolar ATP synthetase putative (PFE0965c), vacuolar ATP synthase subunit F putative (PF11_0412), vacuolar ATP synthase subunit D putative (PF13_0227), vacuolar ATP synthase catalytic subunit a (PF13_0065) - Mitochondrial ATP synthase F1: ATP synthase subunit putative (PF14_0615), mitochondrial ATP synthase F1 epsilon subunit (MAL7P1.75), mitochondrial ATP synthase F1 alpha subunit putative (PFB0795w). Red and blue colors correspond respectively to up- and down-regulated genes compared between hyperoxic to normoxic conditions.