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Table 2 Proteins identified by differential 2-D DIGE analysis following hyperoxia exposure of P. falciparum

From: Global response of Plasmodium falciparum to hyperoxia: a combined transcriptomic and proteomic approach

gi numberc

Gene ID

Protein name

MW (kDa)

pI

Master spot number

Significance (Mascot score)

Average ratio

t-Test

P. falciparum

Translation

gi|124512420

MAL8P1.69

14-3-3 protein homologue

29.86

4.96

3289

425

-1.54

0.0078

gi|124513850

PF13_0304

elongation factor 1 alpha

49.16

9.12

2047

130

1.60

0.0090

gi|8918238

PF14_0486

elongation factor 2

85.03

6.30

1030

67

1.72

0.0070

gi|124810293

PF14_0655

RNA helicase-1

45.62

5.48

2268

105

1.53

0.040

Parasitophorous vacuolar membrane Transporter

gi|124810348

PF14_0678

exported protein 2

33.62

5.10

3061

88

-1.92

0.00093

     

3062

180

-2.06

0.0046

Glycolysis

gi|124809201

PF14_0341

glucose-6-phosphate isomerase

67.61

6.78

1579

36

-1.63

0.00092

gi|124810131

PF14_0598

glyceraldehyde-3-phosphate dehydrogenase

37.08

7.59

2867

695

-1.69

0.033

Chaperone-assisted protein folding

gi|124512406

PF08_0054

heat shock protein 70

74.39

5.51

1314

257

1.65

0.03

     

1326

137

1.58

0.026

gi|505340

PF07_0029

heat shock protein 86

86.77

4.91

1028

412

1.50

0.0052

Amino acids metabolism

gi|86170756

PFF0435w

ornithine aminotransferase

47

6.47

2217

96

-2.14

0.0081

gi|124513590

MAL13P1.214

phosphoethanolamine

31.31

5.43

3347

145

-2.05

0.0055

  

N-methyltransferase

  

3372

252

-1.96

0.00089

     

3379

253

-2.17

0.00058

     

3503

52

-1.62

0.00056

     

3385

452

-2.71

0.00026

Proteasome-mediated proteolysis

gi|124512686

MAL8P1.142

proteasome beta-subunit

31.08

6.00

3216

58

1.78

0.015

gi|124513790

MAL13P1.270

proteasome subunit

27.50

6.17

3358

142

1.55

0.049

Homo sapiens

Oxygen transporter

gi|183817

 

Beta-globin

19.21

6.28

3451

165

7.71

0.020

     

3576

102

1.91

0.011

Antioxidant metabolism

gi|4502517

 

carbonic anhydrase I

28.91

6.59

3431

173

4.18

0.0095

     

3444

155

3.47

0.0019

     

3455

224

4.02

0.00038

gi|4557014

 

catalase

59.95

6.9

1648

200

6.47

0.012

gi|16306550

 

selenium binding protein 1

52.93

5.93

2451

73

-1.64

0.041

gi|168985379

 

flotillin 1

39.81

6.03

2156

157

1.64

0.040

Glycolysis

gi|31645

 

glyceraldehyde-3-

36.20

8.26

2804

199

-1.94

0.0042

  

phosphate dehydrogenase

  

2806

181

-1.99

0.012

     

2807

132

-1.84

0.0069

     

2853

112

-2.17

0.0027

     

2854

122

-2.25

0.0032

     

2856

96

-2.20

0.0019

  1. The proteins were identified by mass spectrometry following in gel trypsin digestion. The spot numbers correspond to the same numbers in figure 4. The Mascot gi number of the spots, their gene ID (PlasmoDB), their name, the theoretical MW and pI values, as well as the corresponding Mascot score are listed for MS/MS analysis (scores greater than 29 for P. falciparum and 38 for Homo sapiens are considered significant (p ≤ 0.05)). Paired average volume ratio (hyperoxic versus normoxic conditions) and p-values (t-Test) were obtained using Decyder software. MW: molecular weight.