Table 1 Primers used for pfcrt sequencing and real-time PCR quantification
Exon 1-2 | Â |
Primary | Â |
   E1/2-F | CGACATTCCGATATATTATATTTTTAGAC |
   E1/2-R | TATATGTGTAATGTTTTATATTGG |
Nested | Â |
   E1/2-NF | CCGTTAATAATAAATACACGCAG |
   E1/2-NR | AATGTTTTATATTGGTAGGTGG |
Exon 3-8 | Â |
Primary | Â |
   E3/8-F | CCACCTACCAATATAAAACATTAC |
   E3/8-R | GTTAAAATATATATAAATGTCTC |
Nested | Â |
   E3/8-NF | TATATATATATGTATGTATGTTG |
   E3/8-NR | AATGTCTCTTATAATTTTGAAATT |
Exon 9-13 | Â |
Primary | Â |
   E9/13-F | CTTATAATAAAATTTCAAAATTATAAGAGAC |
   E9/13-R | GAGATCTCTATACCTTCAACATTATTCC |
Nested | Â |
   E9/13-NF | GAGACATTTATATATATTTTAAC |
   E9/13-NR | CCTTATAAAGTGTAATGCG |
pfcrt RT-PCR | Â |
pfcrt-F | CTTTATTTGTATGATTATGTTCTTTATTGTTTATTCCTTATTTGGA |
pfcrt-R | AACAGGCATCTAACATGGATATAGCAAA |
pfcrt-probe | TCGGTGTCGTTCTTTTG |
pfmdr RT-PCR | Â |
pfmdr-F | GCATTCGGTTTTTGGTATGGTACAA |
pfmdr-R | CCTAATAAAATGGATATAACTGAGGCACCA |
pfmdr-probe | ACGAATCAATACCCCAATAAT |
EF-1a RT-PCR | Â |
EF-1a-F | GGCAGAAAGAGAAAGAGGTATTACCA |
EF-1a-R | CCTGGTGCATCAATGACAGTAAAGA |
EF-1a-probe | TTATGGAAATTTGAAACCCC |