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Table 1 PCR amplification success of DNA extracted from different types/brand of archived RDTs

From: Using rapid diagnostic tests as source of malaria parasite DNA for molecular analyses in the era of declining malaria prevalence

Category of RDTs sampled

No. of RDTs

Type/brand of RDT

Mean storage duration in days(range)

PCR results

    

Positive (%)

Negative (%)

True positive: RDT +ve/BS +ve

26

Paracheck Pf®

28(7-58)

26(100)

0(0)

True positive: RDT +ve/BS +ve

45

ParaHIT ®f

47(6-95)

44(97.8)

1(2.2)

False negative: RDT -ve/BS +ve

63

ParaHIT ®f

60(61-66)

52(82.5)

11(17.4)*

False positive: RDT +ve/BS -ve

24

ParaHIT ®f

60(55-66)

4(16.7)

20(83.3)

True negative: RDT -ve/BS -ve

7

ParaHIT ®f

65(63-69)

0(0)

7(100)

  1. RDT = Rapid Diagnostic Test, BS = Blood smear, +ve = positive and -ve = negative
  2. *These samples had low parasite density detected by microscopy (less than 100 asexual parasites/μ; ranged from 16-80 asexual parasites/μl).