Table 1 Name, sequence and amplicon sizes generated by Plasmodium falciparum msp1, msp2 and glurp PCR amplification primers
From: Plasmodium falciparum msp1, msp2 and glurp allele frequency and diversity in sub-Saharan Africa
Gene | Amplification | Primer | Primer sequence |
|---|---|---|---|
msp1 | Primary | M1OR | 5'cttaaatagtattctaattcaagtggatca 3' |
| Â | Â | MIOF | 5'ctagaagctttagaagatgcagtattg 3' |
| Â | Secondary | MKR | 5'gcttgcatcagctggagggcttgcaccaga 3' |
| Â | Â | MKF | 5'aaatgaagaagaaattactacaaaaggtgc 3' |
| Â | Secondary | M1MR | 5'atctgaaggatttgtacgtcttgaattacc 3' |
| Â | Â | M1MF | 5'aaatgaaggaacaagtggaacagctgttac 3' |
| Â | Secondary | M1RF | 5'taaaggatggagcaaatactcaagttgttg 3' |
| Â | Â | RO33R2 | 5'caagtaattttgaactctatgttttaaatcagcgta 3' |
msp2 | Primary | S2 | 5'-gaaggtaattaaaacattgtc 3' |
| Â | Â | S3 | 5'-gagggatgttgctgctccacag 3' |
| Â | Secondary | S1TAIL FW | 5'-gcttataatatgagtataaggagaa 3' |
| Â | Â | M5-FC27-RV | 6FAM 5'-gcattgccagaacttgaa 3' |
| Â | Â | N5-3D7-RV | VIC 5'-ctgaagaggtactggtaga 3' |
glurp | Primary | GF3 | 5'acatgcaagtgtgatcctgaa 3' |
| Â | Â | GF4* | 5'tgtaggtaccacgggttcttgtgg 3' |
| Â | Secondary | GNF | 5'tgttcacactgaacaattagatttagatca 3' |