Figure 4
From: Intracellular proteolysis of kininogen by malaria parasites promotes release of active kinins
SDS-PAGE analysis of biotin-HK hydrolysis by falcipain-2 and falcipain-3. Biotin-HK (0.4 μM) was incubated with each falcipain protease (0.2 μM) for 1 h, and the individual components (HK, FP-2, FP-3) and combined reactions were then separated by SDS-PAGE and their products visualized by nitrocellulose transblots staining with streptavidin–peroxidase conjugated (1:1000/1 h) and its substrate 4-Chloro-1-naphthol.