Table 1 Correlation of the four strategies employed for gating raw flow cytometry data
Manual gating | ||||
|---|---|---|---|---|
Gabonese adults (n = 37)a | MFI day 0 | MFI day 84 | PPFC day 0 | PPFC day 84 |
k-means | r = 0.95 | r = 0.89 | ρ = 0.04§ | ρ = 0.14§ |
r2 = 0.91 | r2 = 0.79 | |||
EM* | r = 0.92 | r = 0.89 | ρ = 0.89 | ρ = 0.94 |
r2 = 0.85 | r2 = 0.80 | |||
Overlap subtraction | r = 0.99 | r = 0.98 | ρ = 0.99 | ρ = 0.99 |
r2 = 0.99 | r2 = 0.96 | |||
Gabonese children (n = 28) b | MFI day 0 | MFI day 84 | PPFC day 0 | PPFC day 84 |
k-means | r = 0.71 | r = 0.76 | ρ = −0.93 | ρ = −0.88 |
r2 = 0.51 | r2 = 0.59 | |||
EM* | r = 0.61 | r = 0.64 | ρ = 0.78 | ρ = 0.81 |
r2 = 0.38 | r2 = 0.41 | |||
Overlap subtraction | r = 0.79 | r = 0.83 | ρ = 0.94 | ρ = 0.96 |
r2 = 0.62 | r2 = 0.69 | |||