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Table 2 Bland-Altman analyses of the different data gating strategies

From: A flow cytometry-based workflow for detection and quantification of anti-plasmodial antibodies in vaccinated and naturally exposed individuals

 

Manual gating

Gabonese adults (n = 37)a

MFI day 0

MFI day 84

PPFC day 0

PPFC day 84

k-means

611

600.9

−0.04

−0.06

 

(464.3, 757.7)

(350.5, 851.3)

(−0.09, 0.005)

(−0.12, -0.005)

Expectation

−388.3

−328.7

0.02

0.01

Maximization

(−600.2, -176.4)

(−604.8, -52.7)

(0.004, 0.04)

(−0.003, 0.03)

Overlap subtraction

−124.2

−98.1

0.006

−0.004

 

(−160.3, -88.2)

(−143.4, -52.7)

(0.003, 0.009)

(−0.02, 0.001)

Gabonese children (n = 28) b

MFI day 0

MFI day 84

PPFC day 0

PPFC day 84

k-means

198.6

205.8

−0.4

−0.34

 

(166.7, 230.6)

(176.8, 234.8)

(−0.49, -0.31)

(−0.42, -0.27)

Expectation

−208.9

−171.7

0.09

0.06

Maximization

(−290.4, -127.3)

(−214.5, -128.9)

(0.03, 0.14)

(0.05, 0.08)

Overlap subtraction

39.8

56.2

−0.04

−0.05

 

(25.1, 54.5)

(43.6, 68.9)

(−0.03, -0.05)

(−0.06, -0.03)

  1. Ab reactivity is expressed as mean fluorescence intensity (MFI) and percentage of positive fluorescent cell (PPFC). Data is given as mean differences of MFI and PPFC values (lower and upper 95% confidence interval) between the different approaches. aData excluded for 3 participants due to problems with data acquisition and inability of some algorithms to set an appropriate gate. bTwo participants have been excluded from analysis for the same reasons as above.