Figure 5

Effect of proteasome inhibitors on mefloquine-induced luciferase activity decrease. Plasmodium falciparum 3D7 parasites expressing firefly luciferase were incubated for 6 hours with 100 nM mefloquine, 5 μM lactacystin or 300 nM MG-132, or with 100 nM mefloquine combined with lactacystin or MG-132 in a 96-well plate. Luciferase activity in drug-exposed (solid bars) and solvent control (shaded bars) cultures was determined with a luminescence based Bright-Glo® Luciferase Assay System reagent (Promega). Individual data-points are mean luminescence values (relative light units – RLU) from three replicate wells and error bars indicate standard deviation.