Detection of P. falciparum infected RBCs using Hoechst staining. (A) Representative flow cytometry fluorescent histogram of RBCs infected with P. falciparum stained with Hoechst. The Hoechst fluorescent axis is in Log scale. (B) RBCs infected with P. falciparum were stained with Hoechst, and then applied to chambered μ-slides. Representative confocal images are shown. Left panels: Differential Interference Contrast (DIC), middle panels: Hoechst (Blue), right panels: overly of Hoechst staining and DIC. Cells positive to Hoechst are marked with yellow arrows. [Red scale bar = 10μm].