Figure 2
Concentration dependent accumulation of Cd in RBCs infected with P. falciparum Cd resistant and sensitive strains. (A-B) Fluo-3/AM fluorescence in Cd concentration dependent manner analysed by flow cytometry, Sensitive and Resistant denote RBCs infected with sensitive and resistant parasite respectively. (A) Two dimensional flow cytometry dot plots of stained populations of RBCs with Fluo-3/AM and Hoechst. (B) After gating the populations for positive and negative to Hoechst, at a threshold of 1.5*103 Hoechst fluorescent intensity, Fluo-3/AM fluorescence geometric mean was calculated. The samples without Cd served as control. RBC-s and RBC-r denote non-infected RBCs originating from the sensitive and resistant cultures respectively. Each time point is a mean of duplicates, Bars ± SD. (C) Confocal images: I: Hoechst (Blue), II: Fluo-3/AM (Green), III: combined Hoechst and Fluo-3/AM, IV: combined Hoechst and Fluo-3/AM with DIC (also shown in the enlarged image). Yellow arrow points to Fluo-3/AM overlapping with Hoechst. [Red scale bar = 20μm]. For the sensitive and resistant strains parasitaemia was 11%. The results are from one representative experiment of three performed.