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Figure 3 | Malaria Journal

Figure 3

From: Dual fluorescent labelling of the human malaria parasite Plasmodium falciparum for the analysis of the ABC type transporter pfmdr2

Figure 3

Time dependent Cd accumulation in RBCs infected with P. falciparum Cd resistant and sensitive strains. (A) Fluo-3/AM fluorescence in a time dependent manner analysed by flow cytometry. After gating the populations for positive and negative to Hoechst, Fluo-3/AM fluorescence geometric mean was calculated. The samples without Cd served as control. Sensitive and resistant denote RBCs infected with sensitive and resistant parasite respectively. RBC-s and RBC-r denote non-infected RBCs originating from the sensitive and resistant cultures respectively. Each time point is a mean of duplicates, Bars ± SD. (B) Confocal images: I: Hoechst (Blue), II: Fluo-3/AM (Green), III: combined Hoechst and Fluo-3/AM, IV: combined Hoechst and Fluo-3/AM with DIC. [Red scale bar = 20μm]. The results are from one representative experiment of two performed. For the sensitive and resistant strains parasitaemia was 10%.

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Malaria Journal

ISSN: 1475-2875

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