Molecular assessment of atpase6 mutations associated with artemisinin resistance among unexposed and exposed Plasmodium falciparum clinical isolates to artemisinin-based combination therapy

Table 1 PCR/RFLP profiles used for genotyping of the pfatpase6 gene

PCR Reaction				RFLP Reaction
Reaction	Primer Sequence (5′→3′)	Ann (Time)	Size	Position	Restriction Enzyme	Product Size
Reaction	Primer Sequence (5′→3′)	Ann (Time)	(bp)	Position		(bp)
Nest-1A	F: TTGGTAATAAAACTCCCGC	58 (2’)	948	-	-	-	-
Nest-1A	R: TATTCCTCTTAGCACCACTCC	58 (2’)	948	-	-	-	-
Nest-2A	F: TCATCTACCGCTATTGTATG	60 (1’)	775	L263E	Apo I	L: 775	E: 633+142
Nest-2A	R: TCCTCTTAGCACCACTCC	60 (1’)	775	E431K	MBoII	E: 299+241+117+69+49	K: 416+241+69+49
Nest-1B	F: AAGAAGGATAAATCACCAAG	55 (1’)	725	-	-	-	-
Nest-1B	R: AAATACACGTATACCAGCC	55 (1’)	725	-	-	-	-
Nest-2Ba	F: TAACCATTCTAATTATACTACAGCg CAGG	60 (1’)	141	A623E	Cac8 I	A: 114+27	E: 141
Nest-2Ba	R: TGTGTTGATGTGGTATTTATTTTATTACCC
Nest-2Bb	F: AGAACAtTTAGCTTTGCTTATAAAAAAc TAA	60 (1’)	164	S769N	DdeI	S: 136+28	N: 164
Nest-2Bb	R: ATATGGCATAATCTAATTGCTCTTCCTAC	60 (1’)	164	S769N	DdeI	S: 136+28	N: 164

ISSN: 1475-2875