Improving N-terminal protein annotation of Plasmodium species based on signal peptide prediction of orthologous proteins

Table 2 Reannotated proteins with direct experimental validation of subcellular localization

PlasmoDB Gene ID	Species	Ortholog group	Signal peptide prediction		ApiLoc*
PlasmoDB Gene ID	Species	Ortholog group	Before	After	Localization	Reference
PF14_0517	Pf	OG4_10729	Negative	Positive	Cytosol and food vacuole during trophozoite	[60]
PFB0400w	Pf	OG4_42799	Positive	Positive	Cytoplasm during gametocyte stage v	[46]
PVX_090075	Pv	OG4_54213	Negative	Positive	Rhoptry during schizont	[45]
PY03011	Py	OG4_48314	Negative	Positive	Apical and basal and not nucleus during salivary gland sporozoite	[61]
PY00454	Py	OG4_21677	Positive	Positive	Microneme during sporozoite	[62]
PY00819	Py	OG4_10672	Negative	Positive	Apicoplast during hepatocyte schizont and salivary gland sporozoite	[63]
PY07092	Py	OG4_47550	Negative	Positive	Apical and not surface during salivary gland sporozoite	[61]
PY04986	Py	OG4_25099	Negative	Positive	Apical during oocyst, sporozoite	[64]

*Recovery of experimental data from the literature, available in the ApiLoc database, describing the localization and timing of expression for reannotated proteins. Species: Pf Plasmodium falciparum, Pv Plasmodium vivax, Py Plasmodium yoelii.

ISSN: 1475-2875