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Figure 2 | Malaria Journal

Figure 2

From: Using CF11 cellulose columns to inexpensively and effectively remove human DNA from Plasmodium falciparum-infected whole blood samples

Figure 2

Percent human DNA (A) and total DNA (B) as estimated by qPCR after leukocyte depletion of blood-parasite mixtures. The volume and parasite density of blood-parasite mixtures were 3 ml and 10,000/μl except where stated otherwise. Three replicates for each filtration method were performed. Dashed lines indicate current criteria for Illumina sequencing: < 50% human DNA and > 500 ng total DNA. LP = Lymphoprep + Plasmodipur, CF11 = filtration using a 5.5-ml CF11 column, CF11 O/N = CF11 filtrate held overnight prior to removal of supernatant, CF11 4 ml = filtration using a 4-ml CF11 column, CF11 low vol = filtration of a 1.5-ml blood-parasite mixture containing 10,000 parasites/μl, CF11 low para = filtration of a 3-ml blood-parasite mixture containing 5,000 parasites/μl, NF = no filtration, NF low para = no filtration of a 3-ml blood-parasite mixture containing 5,000 parasites/μl. Box plots show median and interquartile range.

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