- Open Access
Polysaccharides from the Chinese medicinal herb Achyranthes bidentata enhance anti-malarial immunity during Plasmodium yoelii 17XL infection in mice
© Zhu et al; licensee BioMed Central Ltd. 2012
- Received: 14 December 2011
- Accepted: 20 February 2012
- Published: 20 February 2012
Clinical immunity to malaria in human populations is developed after repeated exposure to malaria. Regulation and balance of host immune responses may lead to optimal immunity against malaria parasite infection. Polysaccharides (ABPS) derived from the Chinese herb ox knee Achyranthes bidentata possess immuno-modulatory functions. The aim of this study is to use the rodent malaria model Plasmodium yoelii 17XL (P. y 17XL) to examine whether pretreatment with ABPS will modulate host immunity against malaria infection and improve the outcome of the disease.
To determine whether ABPS could modulate immunity against malaria, mice were pretreated with ABPS prior to blood-stage infection by P. y 17XL. Host survival and parasitaemia were monitored daily. The effect of pretreatment on host immune responses was studied through the quantitation of cytokines, dendritic cell populations, and natural regulatory T cells (Treg).
Pretreatment with ABPS prior to infection significantly extended the survival time of mice after P. y 17XL infection. At three and five days post-infection, ABPS pretreated mice developed stronger Th1 immune responses against malaria infection with the number of F4/80+CD36+ macrophages and levels of IFN-γ, TNF-α and nitric oxide being significantly higher than in the control group. More importantly, ABPS-treated mice developed more myeloid (CD11c+CD11b+) and plasmacytoid dendritic cells (CD11c+CD45R+/B220+) than control mice. ABPS pretreatment also resulted in modulated expression of MHC-II, CD86, and especially Toll-like receptor 9 by CD11c+ dendritic cells. In comparison, pretreatment with ABPS did not alter the number of natural Treg or the production of the anti-inflammatory cytokine IL-10.
Pretreatment with the immuno-modulatory ABPS selectively enhanced Th1 immune responses to control the proliferation of malaria parasites, and prolonged the survival of mice during subsequent malaria infection.
- Achyranthes bidentata polysaccharides
- Plasmodium yoelii 17XL
- Immune responses
- Immuno modulatory effect
Malaria is a serious infectious disease caused by protozoan parasites of the genus Plasmodium. Worldwide, there are estimated 200-300 million malaria cases annually, causing approximately one million deaths, mainly in children . The fatalities are normally associated with severe malaria involving life-threatening complications such as acute respiratory distress, metabolic acidosis and cerebral malaria. Despite treatment with the best available anti-malarial drugs and supportive care, severe malaria is fatal in 10-30% of patients [2, 3]. Additional adjunctive management strategies are being considered to reduce the severity of disease.
In endemic areas, repeated exposure to malaria infection results in the development of clinical immunity to disease, which is characterized by low parasitaemia levels rather than sterilizing immunity. Therefore, the primary focus of malaria vaccines is to reduce morbidity and mortality associated with this disease. Better understanding of host immunity against malaria is needed to achieve this goal. Immunity to asexual blood stages of malaria parasites involves both cellular and antibody-mediated mechanisms . Although cell-mediated immune responses play the major role in protection against parasite replication, they also result in detrimental inflammation and contribute to induction of severe disease. CD4+ T cells govern the cell-mediated immune response to malaria parasites through the production of cytokines. Pro-inflammatory (Th1) and anti-inflammatory (Th2) cytokines produced by CD4+ T cells play decisive roles in the outcome of malaria infections. Early pro-inflammatory cytokine responses seem to mediate protective immunity, whereas late in infection they appear to contribute to pathology . In mice, the anti-inflammatory cytokine IL-10, produced by natural regulatory T cells (Treg), is required to limit pro-inflammatory immune responses [5–7]. Meanwhile, as the pacemaker of the immune response, dendritic cells (DCs) provide a source of cytokines that contribute to shaping up cell-mediated and humoral immunity by inducing Th1/Th2 differentiation of T cells and antibody production by B cells [8, 9]. In addition to DCs, another important effector molecule in malaria is the toxic free radical NO, which is widely recognized as an important biological mediator . In the context of an acute inflammatory disease, such as malaria, much of the NO produced comes from the cytokine inducible NO synthase (iNOS) present in monocytes, macrophages, and neutrophils . Pro-inflammatory cytokines (e.g. IFN-γ and TNF-α) increase iNOS-generated NO production, whereas anti-inflammatory cytokines (e.g. IL-10 and TGF-β) down-regulate NOS2 expression . NO also has been reported to have potent parasiticidal properties against P. falciparum and can thereby limit parasitaemia . These findings highlight the significance of a crucial balance in the inflammatory response for controlling infection and preventing pathology in malaria. Thus, regulation of host immune responses may lead to enhanced immunity against malaria parasite infection.
Polysaccharides are high molecular weight compounds formed by repeating sub-units of sugars, which are widely distributed in plants, animals and microbes [14–16]. Polysaccharides have been shown to possess anti-tumour, anti-hepatitis and anti-senile effects, as well as immunomodulatory functions, such as stimulating splenocyte proliferation while in combination with LPS, activating macrophage, NK T cells, B cells and maturation of DCs [17, 18]. Ox knee (Achyranthes bidentata) is a traditional Chinese medicinal herb, which has anti-inflammatory activities and is used to "nourish the kidney and liver, drain dampness and promote circulation". The purified A. bidentata polysaccharides (ABPS) are water-soluble molecules with its unit composition molecular weight of ~1400 D, and contain fructose and glucose residues in the molar ratio of 8:1. ABPS are bioactive and have many important functions such as anti-tumour, anti-inflammation and anti-aging [19–22]. The immuno-modulatory effect of ABPS has prompted us to investigate whether pretreatment with ABPS affects the host immunity against malaria infection. Here, the murine malaria model was used with the lethal strain of Plasmodium yoelii 17XL (P. y 17XL). In this model, mice are unable to establish an effective Th1 response and die during the early stages of infection [23, 24]. The results indicated that pretreatment with ABPS could boost host Th1 response against the parasite and prolong the survival of infected mice.
Mice, parasites and experimental infection
Female BALB/c mice, aged six to eight weeks, were purchased from Beijing Animal Institute (Beijing, China). P. y 17XL parasitized erythrocytes were purified as previously described . To initiate malaria infection, each mouse was injected intraperitoneally (i.p.) with 1 × 106P. y 17XL parasitized erythrocytes per mouse. Parasitaemia was monitored by light microscopy. Mortality of infected mice was monitored daily. All experiments were performed in compliance with the local Institutional Animal Care and Use Committee.
White powder ABPS with 99% purity was kindly provided by Gengyuan Tian of Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, Shanghai, China. ABPS injection solutions were prepared with physiological saline. To study the effect of ABPS on parasitaemia and host survival, 0.1 ml ABPs solutions were injected i.p. daily into mice at the dosage of 50 mg/kg for consecutive 10 and 15 days before or immediately after P.y 17XL infection. This dosage was adopted based on an earlier report, which shows that 50 mg/kg of ABPS is optimal against Lewis lung cancer in C57BL/6 mice . The control group received the same volume of physiological saline at the same time points. Subsequently, to study the immuno-modulatory effect of ABPS, two dosages of ABPS (10 and 50 mg/kg) were used for i.p. injection daily into the mice for consecutive 10 days before infection [22, 26].
Splenocyte preparation, culture and measurement of cytokines and nitric oxide
Splenocyte culture was performed as previously described . Briefly, spleens from uninfected and infected mice were removed aseptically and pressed through a sterile fine-wire mesh with 10 ml RPMI 1640 supplemented with 10% heat-inactivated foetal calf serum (FCS), 25 mM Hepes, 0.12% gentamicin and 2 mM glutamine. Cell suspensions were collected by centrifuging at 350 g for 10 minutes. Cell viability was determined by Trypan Blue exclusion and was > 90%. Aliquots of the cell suspensions containing 5 × 106 cells in 500 μl were incubated in 24-well flat-bottom tissue culture plates in triplicate for 48 hours at 37°C in a humidified 5% CO2 incubator. Supernatant fractions were collected and stored at -80°C. Levels of IFN-γ, TNF-α and IL-10 were measured by commercial enzyme-linked immuno-sorbent assay (ELISA) kits according to the manufacturer's protocol (R&D Systems). As a measure of nitric oxide (NO) production, concentrations of NO2- in cell supernatants were determined by the Griess reaction .
Flow cytometry analysis
All antibodies used for flow cytometry were purchased from BD Biosciences or eBioscience (San Diego, CA, USA) unless otherwise indicated. To assess natural Treg, spleen cells were double-stained with FITC-conjugated anti-CD4 monoclonal antibody (mAb) and PE-conjugated anti-CD25 mAb. After fixation and permeabilization, cells were stained with APC-conjugated anti-Foxp3 mAb. To distinguish the sub-sets of DCs, spleen cells were double-stained with FITC-conjugated anti-CD11c mAb and PE-conjugated anti-CD11b mAb or PerCP-conjugated anti-B220 mAb. To measure the maturation markers of DCs, spleen cells were double-stained with FITC-conjugated CD11c mAb and PE-conjugated anti-CD80, CD86, CD40 or MHC-II mAb. To determine the expression of Toll-like receptor 9 (TLR9) within DCs, spleen cells were first stained with FITC-conjugated CD11c mAb, fixed, permeabilized and probed with biotinylated anti-TLR9 mAb followed by PE-conjugated streptavidin. Macrophages were analysed by staining spleen cells with FITC-conjugated F4/80 mAb and/or PE-conjugated anti-CD36 mAb. After staining, the cells were washed twice with PBS containing 1% foetal calf serum and suspended in 500 μl of PBS. All cells were analysed by flow cytometry using a FACSCalibur™ flow cytometer with the CellQuest software version 3.3 (BD Biosciences, Franklin Lakes, NJ, USA). Viable cells were gated by forward and side scattering [28, 29].
Statistical significance of the results was analysed by one-way ANOVA. Time-to-event data were statistically analysed with the Kaplan-Meier approach to survival analysis (SPSS 17.0). A value of P < 0.05 was considered significant.
ABPS extends survival of Plasmodium yoelii 17XL infected mice
ABPS have immuno-regulatory effects on adaptive immune responses
ABPS do not affect the response of natural Treg cells during Plasmodium yoelii 17XL infection
ABPS increase TLR9 expression in CD11c+ DCs
The Chinese herbal medicine ox knee (A. bidentata) possesses pro-inflammatory activities, and polysaccharides from this herb have been shown to enhance immunity . This study demonstrated that pretreatment with ABPS could delay the progression of malaria in mice, probably through boosting of host immunity. It was also found that ABPS could effectively control parasitaemia levels and extend host survival. ABPS treatment enhanced the production of IFN-γ, TNF-α and NO, and increased the number of F4/80+CD36+ macrophages. ABPS also promoted the differentiation and activation of DCs with increased expression of maturation markers. This study further verified the immuno-modulatory activity of ABPS during the early stage of P.y 17XL infection in this murine malaria model, which provided some insights into the regulatory mechanisms by which ABPS mediate anti-malarial protection.
During malaria infection, effective Th1 immune responses at the early stages of infection critically influence the later development and final outcome of the disease [31–34]. IFN-γ is found to be necessary during the resolution of primary infection and for limiting parasite replication in early phase of the infection [35, 36]. In current study, it was found that pretreatment with ABPS significantly stimulated IFN-γ, TNF-α and NO production in vivo during P.y 17XL infection, which might be responsible for the observed improvement in the extended survival in ABPS-treated mice.
DCs provide a critical link between the innate and adaptive immune responses. Increased expression of co-stimulatory molecules, which is characteristic of maturation of DCs, is crucial to the activation of T cells . These molecules have been shown to play an important role in murine malaria models. High expression of MHC-II molecules is crucial for DCs to present antigens to CD4+ T cells. It has been found that blocking the CD80/CD86 signaling pathway disrupts the Th1/Th2 balance in the Plasmodium chabaudi AS malaria model . It is reported that ABPS can induce murine DCs phenotypic maturation in vitro as revealed by increased expression of CD86, CD40 and MHC-II . In this study, the significant up-regulation of co-stimulatory molecules on the surface of CD11c+ cells in ABPS-treated mice was detected, indicating that ABPS treatment could significantly improve the maturation of DCs.
TLRs are expressed on or within innate immune cells including DCs, and recognize pathogen-associated molecular patterns from different microorganisms . Accumulating evidence suggests that TLRs are also involved in both rodent and human malaria [40–42]. It has been reported that TLR9 responds to haemozoin and parasite protein-DNA complex released from the parasitized erythrocytes [41, 43, 44], and TLR9 polymorphisms are associated with disease manifestation in malaria . The significantly elevated expression of TLR9 in DCs in P.y 17XL-infected mice pretreated with ABPS suggests that TLR9 might be essential for ABPS-mediated immuno-regulation during malaria infection.
Increasing evidence indicates that innate immune responses contribute to the control of blood-stage malaria infection, reduced parasite burden, and slowing down of the progression to severe disease. Macrophages have a remarkable non-specific ability to phagocytize and kill protozoan parasites. Both human and rodent macrophages effectively phagocytize P.y 17XL-parasitized erythrocytes [45, 46]. Furthermore, the class B family of scavenger receptor CD36 on macrophages is also implied in protection against malaria, since CD36-deficient individuals are at a greater risk of developing severe and cerebral malaria . Whereas the total number of macrophages (F4/80+) in ABPS-pretreated mice was relatively unchanged, the number of F4/80+CD36+ macrophages significantly increased at 3 dpi in ABPS pretreated mice. As such, the increase in the number of CD36+ macrophages might be involved in phagocytosis of parasitized erythrocytes, leading to reduced parasitaemia at 5 dpi in ABPS-pretreated mice.
Natural Treg expand during Plasmodium infection [5, 24, 48, 49] and have been shown to inhibit the development of Th1 immune responses [3, 24], leading to persistent rise of parasitaemia [24, 50]. Here it was found that ABPS only improved the activation of DCs, which may improve the initiation of an antigen-specific immune response, whereas no effect was observed on Treg or production of the anti-inflammatory cytokine IL-10. This suggests that the immuno-regulatory effects of ABPS may be selective on boosting different host immune responses.
Taken together, the current study further confirmed the immuno-regulatory effects of ABPS. The results have demonstrated that ABPS could activate immune responses against malaria blood-stage infection in mice and that this effect may be mediated by stimulation of DC maturation and activation of F4/80+CD36+ macrophages, which may phagocytose parasitized erythrocytes and release pre-inflammatory cytokines. These findings laid the foundation for further testing the potential benefits of administering ABPS as an "immuno-prophylaxis" to inhibit preemptively the progression of malaria infection.
The aim of this study is to determine whether pretreatment of hosts with immuno-modulatory polysaccharides from the Chinese medicinal herb ox knee, could affect host immunity during malaria infection in a rodent malaria model. It was found that pretreatment of mice with ABPS could boost host Th1 immune responses during early P.y 17XL infection, which includes elevated numbers of activated macrophages and Th1 cytokines. In addition, ABPS pretreatment promoted the maturation of DCs, which are essential in development of parasite-specific adaptive immunity. Yet, the treatment did not alter the number of Treg and the level of anti-inflammatory cytokine IL-10. Findings from this study underscore the potential of administering immuno-modulatory compounds like ABPS to strengthen host immunity against malaria.
This work was supported by grants from the National Natural Science Foundation of China (30800962). We are grateful to all other staff in the College of Animal Science and Technology.
- WHO: World Malaria Report. 2010, [http://www.who.int/malaria/world_malaria_report_2010/en/index.html]Google Scholar
- Day N, Dondorp AM: The management of patients with severe malaria. Am J Trop Med Hyg. 2007, 77: 29-35.PubMedGoogle Scholar
- Dondorp A, Nosten F, Stepniewska K, Day N, White N: Artesunate versus quinine for treatment of severe falciparum malaria: a randomised trial. Lancet. 2005, 366: 717-725.View ArticlePubMedGoogle Scholar
- Doolan DL, Dobano C, Baird JK: Acquired immunity to malaria. Clin Microbiol Rev. 2009, 22: 13-36. 10.1128/CMR.00025-08.PubMed CentralView ArticlePubMedGoogle Scholar
- Nie CQ, Bernard NJ, Schofield L, Hansen DS: CD4+ CD25+ regulatory T cells suppress CD4+ T-cell function and inhibit the development of Plasmodium berghe-specific TH1 responses involved in cerebral malaria pathogenesis. Infect Immun. 2007, 75: 2275-2282. 10.1128/IAI.01783-06.PubMed CentralView ArticlePubMedGoogle Scholar
- Moore KW, de Waal Malefyt R, Coffman RL, O'Garra A: Interleukin-10 and the interleukin-10 receptor. Annu Rev Immunol. 2001, 19: 683-765. 10.1146/annurev.immunol.19.1.683.View ArticlePubMedGoogle Scholar
- Omer FM, Riley EM: Transforming growth factor beta production is inversely correlated with severity of murine malaria infection. J Exp Med. 1998, 188: 39-48. 10.1084/jem.188.1.39.PubMed CentralView ArticlePubMedGoogle Scholar
- Steinman RM, Hemmi H: Dendritic cells: translating innate to adaptive immunity. Curr Top Microbiol Immunol. 2006, 311: 17-58. 10.1007/3-540-32636-7_2.PubMedGoogle Scholar
- Manicassamy S, Pulendran B: Modulation of adaptive immunity with Toll-like receptors. Semin Immunol. 2009, 21: 185-193. 10.1016/j.smim.2009.05.005.PubMed CentralView ArticlePubMedGoogle Scholar
- Nathan C, Xie QW: Nitric oxide synthases: roles, tolls, and controls. Cell. 1994, 78: 915-918. 10.1016/0092-8674(94)90266-6.View ArticlePubMedGoogle Scholar
- Geller DA, Billiar TR: Molecular biology of nitric oxide synthases. Cancer Metastasis Rev. 1998, 17: 7-23. 10.1023/A:1005940202801.View ArticlePubMedGoogle Scholar
- Rockett KA, Awburn MM, Cowden WB, Clark IA: Killing of Plasmodium falciparu in vitro by nitric oxide derivatives. Infect Immun. 1991, 59: 3280-3283.PubMed CentralPubMedGoogle Scholar
- Kremsner PG, Winkler S, Wildling E, Prada J, Bienzle U, Graninger W, Nussler AK: High plasma levels of nitrogen oxides are associated with severe disease and correlate with rapid parasitological and clinical cure in Plasmodium falciparu malaria. Trans R Soc Trop Med Hyg. 1996, 90: 44-47. 10.1016/S0035-9203(96)90476-9.View ArticlePubMedGoogle Scholar
- Alper J: Searching for medicine's sweet spot. Science. 2001, 291: 2338-2343. 10.1126/science.291.5512.2338.View ArticlePubMedGoogle Scholar
- Helenius A, Aebi M: Intracellular functions of N-linked glycans. Science. 2001, 291: 2364-2369. 10.1126/science.291.5512.2364.View ArticlePubMedGoogle Scholar
- Rudd PM, Elliott T, Cresswell P, Wilson IA, Dwek RA: Glycosylation and the immune system. Science. 2001, 291: 2370-2376. 10.1126/science.291.5512.2370.View ArticlePubMedGoogle Scholar
- Peng ZG, Chen HS, Guo ZM, Dong B, Tian GY, Wang GQ: Anti-HIV activities of Achyranthes bidentat polysaccharide sulfate in vitro and in vivo. Yao Xue Xue Bao. 2008, 43: 702-706.PubMedGoogle Scholar
- Zou Y, Meng J, Chen W, Liu J, Li X, Li W, Lu C, Shan F: Modulation of phenotypic and functional maturation of murine dendritic cells (DCs) by purified Achyranthes bidentat polysaccharide (ABP). Int Immunopharmacol. 2011, 11: 1103-1108. 10.1016/j.intimp.2011.03.006.View ArticlePubMedGoogle Scholar
- Chen Q, Liu Z, He JH: Achyranthes bidentat polysaccharide enhances immune response in weaned piglets. Immunopharmacol Immunotoxicol. 2009, 31: 253-260. 10.1080/08923970802439795.View ArticlePubMedGoogle Scholar
- Deng HB, Cui DP, Jiang JM, Feng YC, Cai NS, Li DD: Inhibiting effects of Achyranthes bidentat polysaccharide and Lycium barbaru polysaccharide on nonenzyme glycation in D-galactose induced mouse aging model. Biomed Environ Sci. 2003, 16: 267-275.PubMedGoogle Scholar
- Han SB, Lee CW, Yoon YD, Lee JH, Kang JS, Lee KH, Yoon WK, Lee K, Park SK, Kim HM: Prevention of arthritic inflammation using an oriental herbal combination BDX-1 isolated from Achyranthes bidentat and Atractylodes japonic. Arch Pharm Res. 2005, 28: 902-908. 10.1007/BF02973875.View ArticlePubMedGoogle Scholar
- Jin LQ, Zheng ZJ, Peng Y, Li WX, Chen XM, Lu JX: Opposite effects on tumor growth depending on dose of Achyranthes bidentat polysaccharides in C57BL/6 mice. Int Immunopharmacol. 2007, 7: 568-577. 10.1016/j.intimp.2006.12.009.View ArticlePubMedGoogle Scholar
- Taylor-Robinson AW: Regulation of immunity to Plasmodiu: implications from mouse models for blood stage malaria vaccine design. Exp Parasitol. 2010, 126: 406-414. 10.1016/j.exppara.2010.01.028.View ArticlePubMedGoogle Scholar
- Wu Y, Wang QH, Zheng L, Feng H, Liu J, Ma SH, Cao YM: Plasmodium yoeli: distinct CD4(+)CD25(+) regulatory T cell responses during the early stages of infection in susceptible and resistant mice. Exp Parasitol. 2007, 115: 301-304. 10.1016/j.exppara.2006.09.015.View ArticlePubMedGoogle Scholar
- Ing R, Segura M, Thawani N, Tam M, Stevenson MM: Interaction of mouse dendritic cells and malaria-infected erythrocytes: uptake, maturation, and antigen presentation. J Immunol. 2006, 176: 441-450.View ArticlePubMedGoogle Scholar
- Lin ZB, Zhang HN: Anti-tumor and immunoregulatory activities of Ganoderma lucidu and its possible mechanisms. Acta Pharmacol Sin. 2004, 25: 1387-1395.PubMedGoogle Scholar
- TT Cao YM, Torii M: Nitric oxide inhibits the development of Plasmodium yoeli gametocytes into gametes. Parasitol Int. 1998, 47: 157-166.Google Scholar
- Grelli S, d'Ettorre G, Lauria F, Montella F, Di Traglia L, Lichtner M, Vullo V, Favalli C, Vella S, Macchi B: Inverse correlation between CD8+ lymphocyte apoptosis and CD4+ cell counts during potent antiretroviral therapy in HIV patients. J Antimicrob Chemother. 2004, 53: 494-500. 10.1093/jac/dkh105.View ArticlePubMedGoogle Scholar
- Seixas E, Ostler D: Plasmodium chabaudi chabau i (AS): differential cellular responses to infection in resistant and susceptible mice. Exp Parasitol. 2005, 110: 394-405. 10.1016/j.exppara.2005.03.024.View ArticlePubMedGoogle Scholar
- Chen G, Liu J, Wang QH, Wu Y, Feng H, Zheng W, Guo SY, Li DM, Wang JC, Cao YM: Effects of CD4(+)CD25(+)Foxp3(+)regulatory T cells on early Plasmodium yoeli 17XL infection in BALB/c mice. Parasitology. 2009, 136: 1107-1120. 10.1017/S0031182009990370.View ArticlePubMedGoogle Scholar
- Malhotra I, Mungai P, Muchiri E, Ouma J, Sharma S, Kazura JW, King CL: Distinct Th1-and Th2-Type prenatal cytokine responses to Plasmodium falciparu erythrocyte invasion ligands. Infect Immun. 2005, 73: 3462-3470. 10.1128/IAI.73.6.3462-3470.2005.PubMed CentralView ArticlePubMedGoogle Scholar
- Othoro C, Moore JM, Wannemuehler KA, Moses S, Lal A, Otieno J, Nahlen B, Slutsker L, Shi YP: Elevated gamma interferon-producing NK cells, CD45RO memory-like T cells, and CD4 T cells are associated with protection against malaria infection in pregnancy. Infect Immun. 2008, 76: 1678-1685. 10.1128/IAI.01420-07.PubMed CentralView ArticlePubMedGoogle Scholar
- Taylor-Robinson AW, Phillips RS: Th1 and Th2 CD4+ T cell clones specific for Plasmodium chabaudi but not for an unrelated antigen protect against blood stage P. chabaudi infection. Eur J Immunol. 1994, 24: 158-164. 10.1002/eji.1830240124.View ArticlePubMedGoogle Scholar
- Taylor-Robinson AW, Phillips RS, Severn A, Moncada S, Liew FY: The role of TH1 and TH2 cells in a rodent malaria infection. Science. 1993, 260: 1931-1934. 10.1126/science.8100366.View ArticlePubMedGoogle Scholar
- McCall MB, Sauerwein RW: Interferon-gamma-central mediator of protective immune responses against the pre-erythrocytic and blood stage of malaria. J Leukoc Biol. 2010, 88: 1131-1143. 10.1189/jlb.0310137.View ArticlePubMedGoogle Scholar
- Su Z, Stevenson MM: Central role of endogenous gamma interferon in protective immunity against blood-stage Plasmodium chabaudi AS infection. Infect Immun. 2000, 68: 4399-4406. 10.1128/IAI.68.8.4399-4406.2000.PubMed CentralView ArticlePubMedGoogle Scholar
- Mockenhaupt FP, Hamann L, von Gaertner C, Bedu-Addo G, von Kleinsorgen C, Schumann RR, Bienzle U: Common polymorphisms of toll-like receptors 4 and 9 are associated with the clinical manifestation of malaria during pregnancy. J Infect Dis. 2006, 194: 184-188. 10.1086/505152.View ArticlePubMedGoogle Scholar
- Taylor-Robinson AW, Smith EC: Modulation of experimental blood stage malaria through blockade of the B7/CD28 T-cell costimulatory pathway. Immunology. 1999, 96: 498-504. 10.1046/j.1365-2567.1999.00718.x.PubMed CentralView ArticlePubMedGoogle Scholar
- Iwasaki A, Medzhitov R: Toll-like receptor control of the adaptive immune responses. Nat Immunol. 2004, 5: 987-995. 10.1038/ni1112.View ArticlePubMedGoogle Scholar
- Coban C, Ishii KJ, Horii T, Akira S: Manipulation of host innate immune responses by the malaria parasite. Trends Microbiol. 2007, 15: 271-278. 10.1016/j.tim.2007.04.003.View ArticlePubMedGoogle Scholar
- Coban C, Ishii KJ, Kawai T, Hemmi H, Sato S, Uematsu S, Yamamoto M, Takeuchi O, Itagaki S, Kumar N, Horii T, Akira S: Toll-like receptor 9 mediates innate immune activation by the malaria pigment hemozoin. J Exp Med. 2005, 201: 19-25. 10.1084/jem.20041836.PubMed CentralView ArticlePubMedGoogle Scholar
- Hisaeda H, Tetsutani K, Imai T, Moriya C, Tu L, Hamano S, Duan X, Chou B, Ishida H, Aramaki A, Shen J, Ishii KJ, Coban C, Akira S, Takeda K, Yasutomo K, Torii M, Himeno K: Malaria parasites require TLR9 signaling for immune evasion by activating regulatory T cells. J Immunol. 2008, 180: 2496-2503.View ArticlePubMedGoogle Scholar
- Wu X, Gowda NM, Kumar S, Gowda DC: Protein-DNA complex is the exclusive malaria parasite component that activates dendritic cells and triggers innate immune responses. J Immunol. 2010, 184: 4338-4348. 10.4049/jimmunol.0903824.PubMed CentralView ArticlePubMedGoogle Scholar
- Gowda NM, Wu X, Gowda DC: The nucleosome (histone-DNA complex) is the TLR9-specific immunostimulatory component of Plasmodium falciparu that activates DCs. PLoS One. 2010, 6: 20398-View ArticleGoogle Scholar
- Patel SN, Serghides L, Smith TG, Febbraio M, Silverstein RL, Kurtz TW, Pravenec M, Kain KC: CD36 mediates the phagocytosis of Plasmodium falciparu-infected erythrocytes by rodent macrophages. J Infect Dis. 2004, 189: 204-213. 10.1086/380764.View ArticlePubMedGoogle Scholar
- Serghides L, Kain KC: Peroxisome proliferator-activated receptor gamma-retinoid X receptor agonists increase CD36-dependent phagocytosis of Plasmodium falciparu-parasitized erythrocytes and decrease malaria-induced TNF-alpha secretion by monocytes/macrophages. J Immunol. 2001, 166: 6742-6748.View ArticlePubMedGoogle Scholar
- Aitman TJ, Cooper LD, Norsworthy PJ, Wahid FN, Gray JK, Curtis BR, McKeigue PM, Kwiatkowski D, Greenwood BM, Snow RW, Hill AV, Scott J: Malaria susceptibility and CD36 mutation. Nature. 2000, 405: 1015-1016. 10.1038/35016636.View ArticlePubMedGoogle Scholar
- Feng H, Zhu X, Qi Z, Wang Q, Wang G, Pan Y, Li Y, Zheng L, Jiang Y, Shang H, Cui L, Cao Y: Transient attenuated Foxp3 expression on CD4(+) T cells treated with 7D4 mAb contributes to the control of parasite burden in DBA/2 mice infected with lethal Plasmodium chabaudi chabaud AS. Scand J Immunol. 2011, 75: 46-53.View ArticleGoogle Scholar
- Vigario AM, Gorgette O, Dujardin HC, Cruz T, Cazenave PA, Six A, Bandeira A, Pied S: Regulatory CD4+ CD25+ Foxp3+ T cells expand during experimental Plasmodiu infection but do not prevent cerebral malaria. Int J Parasitol. 2007, 37: 963-973. 10.1016/j.ijpara.2007.01.004.View ArticlePubMedGoogle Scholar
- Bueno LL, Morais CG, Araujo FF, Gomes JA, Correa-Oliveira R, Soares IS, Lacerda MV, Fujiwara RT, Braga EM: Plasmodium viva: induction of CD4 + CD25 + FoxP3+ regulatory T cells during infection are directly associated with level of circulating parasites. PLoS One. 2010, 5: 9623-10.1371/journal.pone.0009623.View ArticleGoogle Scholar
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