Strain or Plasmid | Relevant phenotype(s) or genotype(s)a | Source/reference |
---|---|---|
PCR4-PbCSP | Apr Kmr, derivative of pCR4Blunt-TOPO (Invitrogen) containing the Plasmodium berghei CSP gene (Figure 1) | This study |
pPA3 | Cmr, pNZ8048 derivative containing c-myc, the acmA Protan (nucleotides 835 to 1492) under control of PnisA, and usp45 ss (PA3) | [15] |
pPA77 | pPA3 derivative producing CSP[Tlong]-Protan | This study |
pPA91 | Apr, pET32C (Novagen) derivative producing CSP[Tlong]-His | This study |
pPA171 | pPA3 derivative producing CSP[2xB]-Protan | This study |
pPA177 | pPA3 derivative producing CSP[Tshort]-Protan | This study |
pPA180 | Mucosis laboratory collection, used for subcloning | This study |
pPA182 | Mucosis laboratory collection, used for subcloning | This study |
pPA193 | Apr, pET32C (Novagen) derivative producing CSP[2xB]-His | This study |
pPA197 | pPA177 derivative producing CSP[2xB-Tshort]-Protan | This study |
pPA198 | pPA177 derivative producing CSP[4xB-Tshort]-Protan | This study |
Lactococcus lactis PA1001 | Derivative of NZ9000 lacking acmA and htrA, allows nisin-inducible expression | [15] |
Escherichia coli BL21(DE3) | Allows IPTG-inducible expression | Novagen |