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Table 1 Bacterial strains and plasmids used in this study

From: Bacterium-like particles as multi-epitope delivery platform for Plasmodium berghei circumsporozoite protein induce complete protection against malaria in mice

Strain or Plasmid

Relevant phenotype(s) or genotype(s)a

Source/reference

PCR4-PbCSP

Apr Kmr, derivative of pCR4Blunt-TOPO (Invitrogen) containing the Plasmodium berghei CSP gene (Figure 1)

This study

pPA3

Cmr, pNZ8048 derivative containing c-myc, the acmA Protan (nucleotides 835 to 1492) under control of PnisA, and usp45 ss (PA3)

[15]

pPA77

pPA3 derivative producing CSP[Tlong]-Protan

This study

pPA91

Apr, pET32C (Novagen) derivative producing CSP[Tlong]-His

This study

pPA171

pPA3 derivative producing CSP[2xB]-Protan

This study

pPA177

pPA3 derivative producing CSP[Tshort]-Protan

This study

pPA180

Mucosis laboratory collection, used for subcloning

This study

pPA182

Mucosis laboratory collection, used for subcloning

This study

pPA193

Apr, pET32C (Novagen) derivative producing CSP[2xB]-His

This study

pPA197

pPA177 derivative producing CSP[2xB-Tshort]-Protan

This study

pPA198

pPA177 derivative producing CSP[4xB-Tshort]-Protan

This study

Lactococcus lactis PA1001

Derivative of NZ9000 lacking acmA and htrA, allows nisin-inducible expression

[15]

Escherichia coli BL21(DE3)

Allows IPTG-inducible expression

Novagen

  1. a) PnisA, nisin-inducible promoter; usp45 ss , signal sequence of usp45