Name | Â | Primer sequence | Annealing | Extension | Cycles |
---|---|---|---|---|---|
asl/outer | fw | GSKAARTTTAATGGKGCTGTWGG | 47°C, 30 s | 72°C, 50 s | 35 |
 | rv | GGATTAAYTTTATGAGGCATTG |  |  |  |
asl/nested | fw | GCTGATMAAAATRTTGATTGG | 50°C, 30 s | 72°C, 30 s | 38 |
 | rv | GAGGCATTGTACTACTWCC |  |  |  |
clpc/outer | fw | AAACTGAATTAGCAAAAATATTA | 50°C, 30 s | 72°C, 50 s | 38 |
 | rv | CGWGCWCCATATAAAGGAT |  |  |  |
clpc/nested | fw | GATTTGATATGAGTGAATATATGG | 48°C, 30 s | 72°C, 30 s | 40 |
 | rv | CCATATAAAGGATTATAWG |  |  |  |
coI/outer | fw | CTATTTATGGTTTTCATTTTTATTTGGTA | 57°C, 30 s | 72°C, 50 s | 35 |
 | rv | AGGAATACGTCTAGGCATTACATTAAATCC |  |  |  |
coI/nested Po | fw | AGCAATATCAATAGCTGCATTACCT | 62°C, 30 s | 72°C, 50 s | 38 |
 | rv | GATTTTCTTCAATATAATGCCTGGA |  |  |  |
cytb/outer | fw | TAATGCCTAGACGTATTCCTGATTATCCAG | 55°C, 30 s | 72°C, 50 s | 35 |
 | rv | TGTTTGCTTGGGAGCTGTAATCATAATGTG |  |  |  |
cytb/nested | fw | TCAACAATGACTTTATTTGG | 55°C, 30 s | 72°C, 50 s | 40 |
 | rv | TGCTGTATCATACCCTAAAG |  |  |  |