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Table 1 Name, sequence and PCR conditions of the primers used

From: The evolutionary host switches of Polychromophilus: a multi-gene phylogeny of the bat malaria genus suggests a second invasion of mammals by a haemosporidian parasite

Name

 

Primer sequence

Annealing

Extension

Cycles

asl/outer

fw

GSKAARTTTAATGGKGCTGTWGG

47°C, 30 s

72°C, 50 s

35

 

rv

GGATTAAYTTTATGAGGCATTG

   

asl/nested

fw

GCTGATMAAAATRTTGATTGG

50°C, 30 s

72°C, 30 s

38

 

rv

GAGGCATTGTACTACTWCC

   

clpc/outer

fw

AAACTGAATTAGCAAAAATATTA

50°C, 30 s

72°C, 50 s

38

 

rv

CGWGCWCCATATAAAGGAT

   

clpc/nested

fw

GATTTGATATGAGTGAATATATGG

48°C, 30 s

72°C, 30 s

40

 

rv

CCATATAAAGGATTATAWG

   

coI/outer

fw

CTATTTATGGTTTTCATTTTTATTTGGTA

57°C, 30 s

72°C, 50 s

35

 

rv

AGGAATACGTCTAGGCATTACATTAAATCC

   

coI/nested Po

fw

AGCAATATCAATAGCTGCATTACCT

62°C, 30 s

72°C, 50 s

38

 

rv

GATTTTCTTCAATATAATGCCTGGA

   

cytb/outer

fw

TAATGCCTAGACGTATTCCTGATTATCCAG

55°C, 30 s

72°C, 50 s

35

 

rv

TGTTTGCTTGGGAGCTGTAATCATAATGTG

   

cytb/nested

fw

TCAACAATGACTTTATTTGG

55°C, 30 s

72°C, 50 s

40

 

rv

TGCTGTATCATACCCTAAAG

   
  1. All denaturation and final extension periods are the same for all primer-pairs.