Table 1 Primers used for pfdhfr amplification

Pfdhfr1(outer forward)

5'-ATG ATG GAA CAA GTC TGC GAC-3'

Pfdhfr2(outer reverse)

5'-C TTG ATA AAC AAC GGA ACC TCC-3'

Pfdhfr3(nested forward)

5'-ACT ACA CAT TTA GAG GTC TAG G-3'

Pfdhfr4(nested reverse)

5'-GG TTC TAG ACA ATA TAA CAT TTA TCC-3'

Pfdhfr5(nested forward)

5'-GCC ATA TGT GCA TGT TGT AAG GTT GAA AG-3'

Pfdhfr6(nested reverse)

5'-CAT ATT TTG ATT CAT TCA CAT ATG TTG TAA CTG CTC-3'