Name | PCR programme |
---|---|
Outer | 94°C 3 min; 5 cycles:94°C 30 sec, 56°C 30 sec, 72°C 45 sec; 8 cycles: 92°C 30 sec, 55°C 30 sec, 72°C 45 sec; 12 cycles: 92°C 30 sec, 53°C 30 sec, 72°C 45 sec; 18 cycles: 92°C 30 sec, 50°C 30 sec, 72°C 45 sec; 16°C hold |
Nested (pfdhfr3+pfdhfr4) | 94°C 3 min; 30 cycles:94°C 1 min, 50°C 1 min, 72°C 1 min; 72°C 10 min; 4°C hold |
Nested (pfdhfr5+pfdhfr6) | 94°C 3 min; 30 cycles: 94°C 1 min, 55°C 1 min, 72°C 1 min; 72°C 10 min; 4°C hold |