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Table 4 Predicted CD8+ T cell-restricted epitopes specific for each volunteer within CSP 15mer peptides

From: Identification of minimal human MHC-restricted CD8+ T-cell epitopes within the Plasmodium falciparum circumsporozoite protein (CSP)

Pool

15mer

Vol.

Predicted epitope

AA no.

IC50nM

HLA allele group

HLA supertype

Epitope no.

Cp1

C3

58

SVSSFLFVEALFQE Y

13-22

258

A*02:01

A02

E1

  

05

SVSSFLFVEALFQEY

15-23

50

A*01:01

A01

E2

  

69

SVSSFLFVEALFQEY

15-23

68

B*35:01

B07

E2

 

C4

12

FLFVEALFQEY QCYG

14-23

226

A*30:02

A01

E3

  

01

FLFVEALFQE YQCYG

13-22

258

A*02:01

A02

E1

  

69

FLFVEALFQEY QCYG

15-23

68

B*35:01

B07

E2

 

C5

58

EALFQEYQCYG SSSN

18-27

2174

A*02:01

A02

E4

 

C6

58

QEYQCYGSSSNT RVL

23-32

11714

A*02:01

A02

E5

Cp2

C8

49

SSNTRVLNELNY DNA

32-40

4075

B*15:03

B27

E6

 

C10

41

ELNYDNAGTNLY NEL

40-48

321

B*35:01

B07

E7

 

C12

49

TNLYNELEM NYY GKQ

45-53

1087

B*15:03

B27

E8

  

12

TNLYNELEMNYY GKQ

47-56

25

A*30:02

A01

E9

  

01

TNLYNELEMNYYG KQ

49-57

468

B*44:02

B44

E10

 

C13

12

NELEMNYYGKQENWY

54-63

132

A*30:02

A01

E11

Cp6

C47

61

DKHIKEYLNKIQNSL

319-327

27

A*02:01

A02

E12

 

C48

41

KEYLNKIQNSL STEW

319-327

27

A*02:01

A02

E12

  

05

KEYLNKIQNSL STEW

319-327

83

B*08:01

B08

E12

  

58

KEYLNKIQNSL STEW

319-327

27

A*02:01

A02

E12

Cp9

C60

61

EKKICKMEKCSSVFN

371-379

2353

B*44:02

B44

E13

 

C62

01

KCSSVFNVVNSSI GL

376-385

470

A*02:01

A02

E14

 

C63

08

VFNVVNSSIGLI MVL

379-388

70

A*68:02

A02

E15

 

C64

08

VNSSIGLIMVL SFLF

382-391

294

A*68:02

A02

E16

 

C65

01

SSIGLIMVLSFLFL N

387-396

53

A*02:01

A02

E17*

  

02

SSIGLIMVLSFLFL N

387-396

53

A*02:01

A02

E17*

  

08

SSIGLIMVLSFLFL N

387-396

816

A*02:01

A02

E17*

  1. The 15mer peptides that were recognized by the volunteers in the initial screen (Table 2) were analysed by NetMHC to predict affinity HLA binding by minimal CD8+ T cell epitopes within each 15-mer (underlined and bold). Those minimal epitopes with the strongest binding affinities for the HLA alleles of each volunteer were selected. Each minimal epitope was specific for a known HLA allele within each supertype. Two listings from Table 4 were not included here because the same minimal peptide was predicted for the same volunteer for two overlapping 15mers (v01, C12 and C13; v05, C3 and C4). Nine of these epitopes were synthesized and further screened by ELISpot with HLA-matched volunteers (see Table 5).
  2. *E17, a 10-mer, was predicted to contain a 9mer sequence LIMVLSFLF labelled E18 (see Table 5).
  3. Vol. = volunteer; AA = amino acid; No. = number.