Skip to main content

Table 7 Summary of predicted and confirmed minimal CSP identified in this study epitopes

From: Identification of minimal human MHC-restricted CD8+ T-cell epitopes within the Plasmodium falciparum circumsporozoite protein (CSP)

15mer peptide

Epitope number

Sequence

AA no.

15mer ELISpot activity

Epitope ELISpot activity

Depl. /ICS

HLA allele group

HLA supertype

Cp1-C3

E1

FLFVEALFQE

13-22

+

+

+

A*02:01

A02

  

FLFVEALFQ E

13-21

   

A*29:02

A01A24

  

FLFVEALF QE

13-20

   

A*29:02

A01A24

 

E2

FVEALFQEY

15-23

+

+

ND

A*01:01

A01

  

FVEALFQEY

15-23

+

+

+

B*35:01

B07

Cp1-C4

E3

LFVEALFQEY

14-23

+

+

ND

A*30:02

A01

Cp9-C62

E14

SVFNVVNSSI

376-385

+

+

ND

A*02:01

A02

     

+

 

A*68:02

A02

  

SVFNVVNSSI

377-385

 

+

+

A*23:01

A24

Cp9-C65

E18

LIMVLSFLF

387-395

 

+

+

A*23:01

A24

     

+

+

A*29:02

A01A24

     

+

 

B*15:03

B27

      

ND

  

Cp1-C1

E19

AILSVSSFLF

6-15

 

+

ND

A*24:03

A24

       

A*23:01

A24

Cp1-C2

E20

SVSSFLFV EA

9-18

 

+

(+)

A*02:01

A02

Cp1-C3

E21

SFLFVEALF

12-20

 

+

+

A*23:01

A24

     

+

+

A*29:02

A01A24

     

+

ND

A*24:02

A24

Cp9-C65

E22

IMVLSFLFL

388-396

 

+

ND

A*02:01

A02

  1. + = Positive activity of the 15mer and predicted epitope in ELISpot assay, or recall of CD8+ T cells in ELISpot depletion (Depl.) assay or intracellular staining/flow cytometry (ICS) assay. (+) = Result not conclusive.
  2. Underlined sequences indicate NetMHC-predicted putative class I-restricted epitopes within E1, E14 and E20.
  3. The five epitopes with + in the Depl./ICS column are confirmed as CD8+ dependent.