Figure 3

Prevalence rates of recurrent infections. Plasmodium parasites were counted against 200 leukocytes with a 100 × objective lens under oil immersion. Each qPCR reaction mixture contained 5 μL DNA template in a final volume of 20 μL, 10 μL of Master Mix (Applied Biosystem), both the genus-specific and P. falciparum specific primers and probes detection system (Plasmo/Pf) as described [24]. Panel A: Prevalence rates of recurrent infections assessed by microscopy (BS) and PCR. Data are shown as pooled (covering the two months following treatment) or split into first and second month (days 7–30 or 31–60). Panel B: Prevalence rates of recurrent infections assessed by PCR according to the IPTp dose received. Data are shown as pooled (covering the two months following treatment) or split into first and second month (days seven-30 or 31–60).