Figure 1From: Plasmodium vivax aldolase-specific monoclonal antibodies and its application in clinical diagnosis of malaria infections in ChinaGene amplification and protein expression. (A) PCR of PvALDO gene. M: DL2000 marker, 1 and 2 represent annealing temperatures 50.4°C and 52.4°C, respectively. (B) Protein expression and purification. M: Marker, lanes 1 and 2 are the whole cell lysate and supernatant after induction, respectively. Lanes 3, 4 and 5 are pooled portions of purified products. (C) Western blot analysis of PvALDO protein. M: Marker, 1: Purified PvALDO protein (10 μg).Back to article page