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Figure 3 | Malaria Journal

Figure 3

From: An alternative protocol for Plasmodium falciparum culture synchronization and a new method for synchrony confirmation

Figure 3

Synchrony comparison of culture synchronization methods. For JS1, parasites were synchronized as outlined in Miao et al.[10]. MS1 parasites were treated similarly to JS1 except sorbitol treatment was omitted. S1 and S2 preparations represent two fractions of the Percoll synchronization method described here. For the unsynchronized culture (US1), schizonts were isolated from a 20% over 60% Percoll cushion. Following synchronization, schizonts were cultured as previously described in 1% haematocrit, and aliquots were taken every 3-hr (starting at 0-hr up to 12-hr). Samples were frozen, thawed, and subjected to SyBr Green I assay. RFI units are listed on the left of the graph. All data points indicate average and standard deviations from triplicate samples.

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