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Figure 1 | Malaria Journal

Figure 1

From: Towards an optimized inhibition of liver stage development assay (ILSDA) for Plasmodium falciparum

Figure 1

Development of P. falciparum in CPHH. A: Representative view of one 40X field typically contains several P. falciparum (Pf) liver schizonts. B: Pf liver stage schizont developing within a CPHH in vitro. Schizonts were stained with mAb anti HSP70 (purchased from LifeSpan Biosciences, Inc.) followed by fluorescein-tagged secondary antibodies (green). Nuclei were stained with DAPI (blue). Scale bar = 20 μm. C: Correlation between qRT-PCR Pf 18s rRNA copy number and counting of HSP70-positive liver stage schizonts was determined by plotting a scatter chart and linear regression (R2) Fifteen data points from 6 separate experiments using sporozoites alone and two data points from two separate experiments using sporozoites mixed with NFS-1 are shown. In all of the samples shown on the graph hepatocytes were analysed 96 hours after sporozoite inoculation. D: Comparison of sporozoite development at 96 hours after 25 k sporozoites inoculated in CPHH and HC04. Error bars represent the standard deviation of the mean.

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