Figure 2From: Towards an optimized inhibition of liver stage development assay (ILSDA) for Plasmodium falciparumFactors influencing P. falciparum liver stage development in CPHH. A: Effect of various sporozoite numbers on infection rate of CPHH. CPHH were inoculated with 25Â k, 50Â k, 75Â k, 100Â k and 140Â k P. falciparum (Pf) sporozoites. The liver stage parasite burden was measured by Pf 18s rRNA qRT-PCR on day 1 (D1) and day 4 (D4). Error bars represent the standard deviation of the mean. B: Effect of centrifugation on invasion rate of CPHH. CPHH were inoculated with 25Â k Pf sporozoites and harvested after 96Â hours. Pf 18s rRNA copy numbers of centrifuged samples were compared to samples without centrifugation. Error bars represent the standard deviation of the mean.Back to article page