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Figure 2 | Malaria Journal

Figure 2

From: Towards an optimized inhibition of liver stage development assay (ILSDA) for Plasmodium falciparum

Figure 2

Factors influencing P. falciparum liver stage development in CPHH. A: Effect of various sporozoite numbers on infection rate of CPHH. CPHH were inoculated with 25 k, 50 k, 75 k, 100 k and 140 k P. falciparum (Pf) sporozoites. The liver stage parasite burden was measured by Pf 18s rRNA qRT-PCR on day 1 (D1) and day 4 (D4). Error bars represent the standard deviation of the mean. B: Effect of centrifugation on invasion rate of CPHH. CPHH were inoculated with 25 k Pf sporozoites and harvested after 96 hours. Pf 18s rRNA copy numbers of centrifuged samples were compared to samples without centrifugation. Error bars represent the standard deviation of the mean.

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