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Table 2 Existing range of in vitro screening methods and in vivo models for blood stage anti-malarials

From: CRIMALDDI: platform technologies and novel anti-malarial drug targets

 

In vitro pharmacology (HTS Screens)

In vivo pharmacology

Human pharmacology

Plasmodium falciparum

•  Radioactive (3H-Hypoxanthine)

Human parasite models:

Human challenge

•  P. falciparum in SCID mouse

•  Colorimetric (pLDH, HRPII)

•  Aotus/Saimiri NHP

Mouse models:

•  Fluorescent (Sybr Green)

•  Plasmodium berghei (including GFP or Luc reporter strains)

•  Transgenic parasite (GFP, Luc reporter strains)

•  Plasmodium vinckei

•  Plasmodium chabaudi

•  Plasmodium yoelii

Plasmodium vivax

Invasion assay

Human parasite models

 

Flow cytometry (both at present only medium throughput)

•  Aotus/ Saimiri NHP

Primate models

•  Plasmodium cynomolgi