From: CRIMALDDI: platform technologies and novel anti-malarial drug targets
In vitro pharmacology | In vitro pharmacology |
---|---|
L/MTS/HTS Screens (using non-transgenic parasites) | MTS/HTS screens (using transgenic parasites) |
Microscopic counts of Giemsa smear (gold standard) | Pfs16-GFP transgenic parasites [33]. |
Flow cytometry of hydroethidine labelled gametocytes [34] | Stage-specific luciferase expression in transgenic parasites [35] |
ATP- bioluminescence assay for stage IV-V gametocytes [36, 37] | Three transgenic GFP lines, used for flow cytometry assay [38] |
Parasite lactate dehydrogenase activity (pLDH) [41] |