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Table 3 Threshold of detection of the real-time PCR screening (including sample volume analysed, DNA extraction and real-time PCR screening): percentage of positive results according to the dilution of parasites

From: An innovative tool for moving malaria PCR detection of parasite reservoir into the field

Dilution (parasites/μl)

% of positive results (No positives/No tested)

 

Assay 1

Assay 2

Average

1,000

100% (8/8)

100% (8/8)

100% (16/16)

100

100% (8/8)

100% (8/8)

100% (16/16)

10

100% (8/8)

100% (8/8)

100% (16/16)

5

ND

100% (8/8)

100% (8/8)

2

ND

100% (8/8)

100% (8/8)

1

87% (7/8)

75% (6/8)

81% (13/16)

0.1

0% (0/8)

0% (0/8)

0% (0/8)

0.01

0% (0/8)

ND

0% (0/8)