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Figure 3 | Malaria Journal

Figure 3

From: Molecular detection and quantification of Plasmodium falciparum-infected human hepatocytes in chimeric immune-deficient mice

Figure 3

Species specificity of primers used for the detection of human and mouse DNA. H: human-specific forward primer, contains the FAM-probe binding site; M: mouse-specific forward primer, contains the Cy5-binding site; H + M: human and mouse common forward primer, used in qPCR experiments. A human and mouse common reverse primer was used in all reactions. Human PBMC’s and SCID mouse liver extract give rise to the human and mouse specific PCR products. The human cell line Huh7.5 does not lead to a human or mouse specific PCR product. Not transplanted mice or low repopulated chimeric mice do not induce a human specific PCR product, whereas a good repopulated chimeric mouse has both human and mouse specific bands. This confirms that the primers used are specific for the mouse and/or human PTGER2 gene expressed in a chimeric mouse liver.

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