Figure 4

Histology and determination of polyploidy of a chimeric liver. (A) A representative section of a chimeric liver (haematoxylin and eosin staining) showing the morphological differences between pale human hepatocytes (upper zone), diseased mouse parenchyma (middle zone) and healthy mouse parenchyma originating from mouse hepatocytes that have eliminated both uPA transgene copies, known as red nodules (lower zone). Detailed view of chimeric liver sections, showing human hepatocytes (B) none of which is multinucleated and mouse hepatocytes (C), one of which is binucleated (arrow). Original magnification A: 200 x, B and C: 400 x.