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Figure 1 | Malaria Journal

Figure 1

From: Cytometric measurement of in vitro inhibition of Plasmodium falciparum field isolates by drugs: a new approach for re-invasion inhibition study

Figure 1

Protocol of measurement of the effect of drugs. A) Life cycle of the parasite: Ring stages of the parasites (R) collected in the blood of patients are cultured for 30 hours without drug to the trophozoite stage (T0). Measurement of inhibition of maturation by a drug occurs during the trophozoite stage (A). It can be detected using radio-labelling of the parasite (RX) or flow cytometry (FC). Inhibition of re-invasion (B) occurs during the schizont (S)/merozoite (M)/ring (R) transition. It can be detected after 24 hours of incubation with drug (C) by flow cytometry (FC), or coupled with inhibition of maturation, after 40 hours of incubation of drug (using either RX or FC methods). B) Labelling of PRCB using hydroethidine; (arrow head: parasites in the erythrocytes).

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