Figure 2

Analysis of parasitized red blood cells using flow cytometry. PRBC are stained with thiazole (FL1) and hydroethidine (FL2). A-B-C/ FL1/FL2 dot plots. Upper right: quarter late stages (T + S: late trophozoites, schizonts); lower right: early stages (R + YT, rings, young trophozoites). A/ asynchronous culture, B/ only young stages of the parasite, C/ same culture 30 hours later (only late stages of the parasite). D/ Automatic analysis of the data using FlowJo software with tracking of the clouds; D1: FL2/FS analysis of hydroethidine-labelled cells (HE+). D2 FL1/FL2 plot with HE + cells gated in D1. HE + TO low: Ring; HE + TO medium: young trophozoite; HE + TO high schizonts and late trophozoites. (T + S)/(R + TY) ratio can be used to calculate inhibition of maturation or re-invasion.