Figure 2
From: Identification of heat shock factor binding protein in Plasmodium falciparum
In vitro characterization of Pf HSBP. (A) 15% SDS-PAGE gel and immunoblot showing purified recombinant Pf HSBP. Lanes:- TL - total lysate, SP - pellet remaining after sonication, SS -supernatant after sonication, FT -flow through, 20Â mM - wash with 20Â mM imidazole, 50Â mM - wash with 50Â mM imidazole, and 200Â mM - elution with 200Â mM imidazole. (B) 2-D-electrophoresis of purified recombinant protein. (C) MS-based identification of Pf HSBP. (D) Circular dichroism spectrum of Pf HSBP.