Figure 7
From: Dihydroisoxazole inhibitors of Anopheles gambiae seminal transglutaminase AgTG3
Inhibition of plugin cross-linking by DHI inhibitors. Fluorescent image of SDS-PAGE for FITC-cadaverine labeling and cross-linking of plugin by AgTG3. Lanes (1–4): (1) AgTG3, (2) Plugin-C, (3) AgTG3 and plugin-C, (4) AgTG3, plugin-C, and 5 mM CaCl2. Lanes (5–8): AgTG3, plugin-C, 5 mM CaCl2 and (5) 10 mM EDTA, (6) 50 mM IA, (7) 100 μM 1, (8) 100 μM 3. (below) Coomassie stain indicating relative levels of monomeric Plugin-C.