Table 1 Annealing temperature for all the primers used
From: Molecular identification of the chitinase genes in Plasmodium relictum
Primer | Seq (as ordered) | Annealing temp. (°C) | |
|---|---|---|---|
PgCHT1_F | Forward | 5’-ATGATAGAAAATCACCAAGACAAATTTTAGA-3′ | 50 |
PgCHT1_R | Reverse | 5’-GGTTCCCAGTCAATATCTACACCA-3′ | 50 |
PgCHT1_F2 | Forward | 5’-TAGAGGAATACAAAAGAAGGAAACAAGG −3′ | 50 |
PgCHT1_R2 | Reverse | 5’-CAGTCAATATCTACACCATCTAAATCA −3′ | 50 |
PgCHT2_F | Forward | 5’-ATTCAAGGTTATTATCCATCATGGGT-3′ | 53 |
PgCHT2_R | Reverse | 5’-GAAATCCTATACAGCTCAAAGCTCC −3′ | 53 |
PgCHT2_F2 | Forward | 5’-GGGTGTCATATAATCATAATATGAAAGA −3′ | 53 |
PgCHT2_R2 | Reverse | 5’-GACATTGATATTAATTTATCCTCACACA −3′ | 53 |
PgCHT1_F3 | Forward | 5’-AATGACTTTGATTTAGATGGTGTAGAT-3′ | 55 |
PgCHT1_R3 | Reverse | 5’-TAATTGTTCTTTCATAAATAAATGCCA −3′ | 55 |
PgCHT2_F3 | Forward | 5’-ATGAACCCAATGGATCGTTTGATG −3′ | 58 |
PgCHT2_R4 | Reverse | 5’-TAAATTATTAGACAAAGACCACAATCC −3′ | 58 |