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Table 1 Oligonucleotide sequence and PCR conditions used to detect Pfcrt K76T SNP

From: Use of proscribed chloroquine is associated with an increased risk of pfcrt T76 mutation in some parts of Ghana

Primer

Sequence (5' - 3')

Size (bp)

PCR condition

Primary amplification

CRT1F

TTGTCGACCTTAACAGATGGCTCAC

526

96°C, 15’ followed by 45X (96°C, 30”; 56°C, 90”; 72°C, 90”); 72°C, 10’

CRT1R

AATTTCCCTTTTTATTTCCAAATAAGGA

 

Nested amplification

CRT2F

CTTGTCTTGGTAAATGTGCTC

200

96°C, 15’ followed by 45X (96°C, 30”; 50°C, 90”; 72°C, 90”); 72°C, 10’

CRT2R

GAACATAATCATACAAATAAAGT