Primer | Sequence (5' - 3') | Size (bp) | PCR condition |
---|---|---|---|
Primary amplification | |||
CRT1F | TTGTCGACCTTAACAGATGGCTCAC | 526 | 96°C, 15’ followed by 45X (96°C, 30”; 56°C, 90”; 72°C, 90”); 72°C, 10’ |
CRT1R | AATTTCCCTTTTTATTTCCAAATAAGGA | ||
Nested amplification | |||
CRT2F | CTTGTCTTGGTAAATGTGCTC | 200 | 96°C, 15’ followed by 45X (96°C, 30”; 50°C, 90”; 72°C, 90”); 72°C, 10’ |
CRT2R | GAACATAATCATACAAATAAAGT |