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Figure 1 | Malaria Journal

Figure 1

From: The development of loop-mediated isothermal amplification targeting alpha-tubulin DNA for the rapid detection of Plasmodium vivax

Figure 1

Primer design for α-tubulin loop-mediated isothermal amplification (LAMP) assay for the detection of Plasmodium vivax. (A) The primers were selected based on a nucleotide sequence alignment of the target region of α-tubulin gene from P. vivax, P. falciparum [GenBank accession no. XM_001351490] and Plasmodium knowlesi [GenBank accession no. XM_002258237] using ClustalW software. Black boxes and bold fonts indicate identical and conserved nucleotides, respectively. The locations of the primer recognition sites are indicated by arrows with the primer names. The black arrowhead indicates the Aat II cleavage site. F3, forward outer primer; B3, backward outer primer; FIP, forward inner primer; BIP, backward inner primer; LF, loop forward primer; and LB, loop backward primer. (B) Sequences of the α-tubulin LAMP primers.

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