Skip to main content

Table 2 Comparison of the results obtained with the diagnostic methods on the 177 whole blood samples

From: The development of loop-mediated isothermal amplification targeting alpha-tubulin DNA for the rapid detection of Plasmodium vivax

Assay

No. positive

No. negative

Sensitivity (95% CId)

Specificity (95% CI)

PPVa(95% CI)

NPVb(95% CI)

% agreement with nested PCR (Kappa)

AUCc(95% CI)

Microscopy

96

81

75.0 (66.8-81.7)

100 (91.1-100)

100 (95.3-100)

60.5 (49.6-70.4)

81.9 (0.624)

0.875 (0.817–0.920)

RDT

137

40

93.0 (87.9–96.4)

63.3 (49.2–75.3)

86.9 (80.1–91.6)

77.5 (62.2–87.8)

84.7 (0.596)

0.781 (0.713–0.840)

Nested PCR

128

49

      

α-tubulin LAMP

137

40

100 (96.4-100)

81.6 (68.3-90.2)

93.4 (87.8-96.6)

100 (89.3-100)

94.9 (0.865)

0.908 (0.856–0.946)

  1. The sensitivities and specificities of the tests were determined using the nested PCR results as gold standard.
  2. aPPV, positive predictive value, bNPV, negative predictive value, cAUC, area under the receiver operating characteristic curve, dCI, confidence interval.