Figure 3From: Detection of antibodies against the CB9 to ICB10 region of merozoite surface protein-1 of Plasmodium vivax among the inhabitants in epidemic areasCharacterization of recombinant merozoite surface protein-1 (ICB910). (A) Purification of recombinant MSP-1 (ICB910) by Ni-NTA agarose affinity chromatography. Lane M, molecular weight marker; lane 1, E. coli DH5α cell lysate after protein expression was induced with IPTG; lane 2, flow-through; lane 3, wash; lane 4, eluted fraction. (B) Western blot analysis of recombinant MSP-1(ICB910) protein. Lanes 1–3, samples from patients infected with malaria; Lanes 4–7, samples from uninfected individuals. (C) Immune responses of recombinant MSP-1(ICB910) to vivax malaria patient group and normal person by Enzyme linked immunosorbent assay.Back to article page