Figure 1

Purified anti-PfRH5 monoclonal antibodies inhibit invasion of P. falciparum strain 3D7. (A) The efficacy of purified anti-PfRH5 monoclonal antibodies (mAbs) to prevent invasion of 3D7 parasites was determined at 0.1 mg/mL. Three mAbs, 2E11, 5A03 and 5A08 were able to inhibit invasion by 98%, 100% and 99%, respectively, while the inhibition of the remaining mAbs ranged from 20-45%. Error bars show standard error. (B) Immunofluorescence assays show these three mAbs with high inhibition recognize native PfRH5 located at the apical end of merozoites. (C) Western blot assay on mature 3D7 parasite lysate with these three purified mAbs also shows that native PfRH5 is recognized by each of these antibodies.