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Figure 3 | Malaria Journal

Figure 3

From: A malaria vaccine candidate based on an epitope of the Plasmodium falciparum RH5 protein

Figure 3

Affinity selection using the anti-PfRH5 mAb 5A08 identifies a single, short linear epitope, AIKK. (A) Agarose gel of purified, unmodified, MS2 VLP (left lane) and a single clone representative of the highly homogenous selectant population from twelve VLPs selected by the anti-PfRH5-5A08 mAb (right lane), stained with ethidium bromide by virtue of the RNA within each VLP. (B) Characterization by sequencing of clones from each round of affinity-selection showed each RH5-5A08 VLP displayed a four-amino acid sequence at its core, AIKK/R, and that by round two, only VLPs with the AIKK epitope were selected for. The full length PfRH5 sequence (from PlasmoDB, gene ID PF3D7_0424100) contains a 4-amino acid identity to the AIKK sequence that occurs only once near the PfRH5 N-terminus (amino acids 28–31, indicated with asterisks), highlighting the potential importance of this epitope with binding to the RBC receptor during parasite invasion.

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