Skip to main content

Table 1 Gene-specific primers and conditions used for dsRNA synthesis

From: Mosquito Akirin as a potential antigen for malaria control

Gene (accession number)

Upstream/downstream primer sequence (5’-3’)

Fragment size (bp)

PCR annealing conditions

 

TAATACGACTCACTATAGGGTACTTTGGCAGTCGTTGTAGTTGC

  

Mosquito akirin (AGAP006809)

 

509

52°C/1 min

 

TAATACGACTCACTATAGGGTACTCACCTGCTTGAAGGTGAACA

  
 

TAATACGACTCACTATAGGGAGACACCCCCACTGAGACTGATACA

  

Mouse B2m (NM_009735)

 

447

62°C/45 sec

 

TAATACGACTCACTATAGGGAGACACCCCCACTGAGACTGATACA

  
  1. T7 promoter sequences (5’-TAATACGACTCACTATAGGGTACT-3’) were included at the 5’-end for dsRNA synthesis.
Back to article page

Malaria Journal

ISSN: 1475-2875

Contact us