Re-evaluation of microscopy confirmed Plasmodium falciparum and Plasmodium vivax malaria by nested PCR detection in southern Ethiopia

Table 1 Species-specific primers used for nested PCR and sequencing

Malaria species	Primer	Primer sequence 5′-3′	Size (bp)
Plasmodium	rPLU5	CCTGTTGTTGCCTTAAACTTC	1,100
Plasmodium	rPLU6	TTAAAATTGTTGCAGTTAAAAC	1,100
P. falciparum	rFAL1		250
P. falciparum	rFAL2	ACACAATGAACTCAATCATGACTACCCGTC	250
P. vivax	rVIV1	CGCTTCTAGCTTAATCCACATAACTGATAC	120
P. vivax	rVIV2	ACTTCCAAGCCGAAGCAAAGAAAGTCCTTA	120
P. malariae	rMAL1	ATAACATAGTTGTACGTTAAGAATAACCGC	144
P. malariae	rMAL2	AAAATTCCCATGCATAAAAAATTATACAAA	144
P. ovale	rOVA1	ATCTCTTTTGCTATTTTTTAGTATTGGAGA	800
P. ovale	rOVA2	GAAAAGGACACATTAATTGTATCCTAGTG	800

ISSN: 1475-2875