Figure 4From: Lambda-carrageenan treatment exacerbates the severity of cerebral malaria caused by Plasmodium berghei ANKA in BALB/c miceAssessment of vascular leakage at the blood–brain barrier by use of Evans blue dye perfusion. Two hundred microliters of 1% Evans blue dye was injected into the tail veins of each mice on day 5 post-infection. After 1 h, the mice were sacrificed using terminal isoflurane anesthesia and the brains were collected. Representative brains are shown: (A) Normal healthy mouse (N), (B) Carrageenan-treated mouse (CGN), (C) P. berghei ANKA-infected, untreated mouse (PbN), (D) P. berghei ANKA-infected, carrageenan-treated mouse (PbCGN). (E) The mean absorbances at 600 nm wavelength and standard deviations of extracted dye after placing the brains in 4% paraformaldehyde for 48 h are shown for each group. Statistically significant differences in mean absorbances were determined by using Tukey’s multiple comparison test (*: P value < 0.05, ns: not significant).Back to article page